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. 2023 Jan 20;7(2):e0018. doi: 10.1097/HC9.0000000000000018

FIGURE 3.

FIGURE 3

Prom1-expressing biliary progenitor cells and their lineage re-populate the EHBD surface epithelium after the murine BA model of RRV injection. (A) Prom1 Cre/+-GFP +/− mice from a BALB/c background were injected with tamoxifen on DOL0. Saline or RRV injection were performed on DOL3. EHBDs were collected at DOL7, DOL13, and DOL21. (B) GFP is limited to the PBGs in the saline group. Surface epithelial GFP expression increases in RRV as time from injury progresses. Scale bar, 50 μm. (C) Surface GFP expression is statistically higher in RRV injured versus saline control at all time points except postinjection day 4 (n=3 biological replicates at each time point). (D) RRV injury was replicated in Prom1 Cre/Cre-GFP +/− (Prom1 KO) mice. GFP is limited to the PBGs in both saline and injury groups. Surface epithelial GFP expression is limited in the luminal epithelium as time from injury progresses in the RRV group. Scale bar, 50 μm. Abbreviations: BA, biliary atresia; EHBD, extrahepatic bile duct; GFP, green fluorescent protein; PBG, peribiliary gland; Prom1, Prominin-1; RRV, rhesus rotavirus.