FIGURE 7.

Null mutation of Prom1 results in decreased organoid motility and growth dynamics. (A) Both Prom1 ^WT and Prom1 ^KO organoids were grown in culture and live imagined for 14 days. Time-lapse videos were made, and organoids were tracked with through time. Organoid track surface area was then graphed over time (blue line) and compared with a normalized surface area line (red line). Size oscillations were recorded (green rectangles). Organoid size oscillation examples are demonstrated in both Prom1 ^WT and Prom1 ^KO organoid tracks. Scale bar, 100 μm. Images of organoids are high-resolution and zoomed-in stills from Supplemental Video 4. (B) Prom1 ^WT organoids traveled nearly twice as far from their starting positions as compared with Prom1 ^KO. (C) Prom1 ^WT organoids also traveled at faster speeds than Prom1 ^KO. (D) Prom1 ^WT organoids oscillated more per track than Prom1 ^KO. (E) Prom1 ^WT organoids grew to larger surface areas during track development than Prom1 ^KO.