FIG 3.

Predicted interaction between AF derivatives and complex II. (A) The position of D5 and a flutolanil derivative (NN23) were merged to predict the interaction between D5 and amino acid residues of E. multilocularis complex II based on the cocrystal structure of A. suum complex II with NN23. (B) The 4-OH in D5 may contribute to forming hydrogen bonds with both Tyr107D and Trp197B. Hydrogen bonds are represented as red dotted lines. The red double arrow shows the electrostatic interactions between the guanidino group of Arg76C and the benzene ring of D5. The benzene group of Phe73C, which is not conserved in A. suum and porcine complex II, might stabilize the binding of D5 by forming hydrophobic interactions with the linker shown as an orange double arrow and red rings.