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. Author manuscript; available in PMC: 2023 Mar 16.
Published in final edited form as: Ann Oncol. 2021 Oct 13;33(1):42–56. doi: 10.1016/j.annonc.2021.09.021

Figure 1. T-cell frequency is increased in tumors.

Figure 1.

(A, B) Representative image of immune cell infiltration in uninvolved lung tissue and tumor analyzed using multiplex immunofluorescence (mIF, A), and quantification of CD3+ cells (n = 139, B). (C) T-cell receptor sequencing examining the T-cell fraction in uninvolved lung tissue and tumor as defined by immunoSEQ (n = 55). (D-I) Percentages of T lymphocytes within CD45+ cells in uninvolved lung tissue and tumor, n = 58, as measured by flow cytometry. (D) Percentage of CD3+ T cells. (E) Percentage of CD8+ T cells. (F) Percentage of CD4+ T cells. (G) Percentage of CD4+CD25hiFoxP3+ Tregs. (H) Ratio of CD8/Tregs from uninvolved lung tissue and tumor. (I) Percentages of proliferating (Ki-67+) CD4+ and CD8+ T cells in uninvolved lung tissue and tumor. Differences between the two groups were calculated with a signed-rank (B-H) or one-way analysis of variance (I).