FIG 4.

Growth of representative FarR variant strains in TSB + 200 μM LA. Cultures were inoculated to OD₆₀₀ = 0.01 into 96-well microtiter plates containing 200 μL of TSB supplemented with a subinhibitory concentration of 200 μM LA + 0.1% DMSO. Plates were incubated at 37°C with orbital shaking, and growth (OD₆₀₀) was monitored hourly. (A) FarR^C116Y (M1545, NM0256, NM0256) and FarR^E160G (hp.082 and hp.099) strains. (B) FarR^E93EE (M1516) and FarR^P165L (DAR1157, DAR948, NM0135) strains. Growth was compared to the ST-5 HA-MRSA (health care-associated MRSA) reference strain N315 and FAR7 (FarR^H121Y). Each data point represents the mean ± standard error of the mean (SEM) from 6× 200-μL wells in 96-well microtiter plates.