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. 2023 Mar 16;3(3):444–458. doi: 10.1158/2767-9764.CRC-22-0480

FIGURE 3.

FIGURE 3

PLXNB1P1597L increases metastasis whereas PLXNB1WT expression suppresses metastasis in Ptenfl/flKrasG12V mouse models of prostate cancer (see also Supplementary Fig. S3–S5). A, Schematic diagram of crosses performed. B, Percentages of animals affected/not affected by metastasis in Ptenfl/flKrasG12V cohorts. Following necropsy, mice were categorized according to their metastatic outcome: no metastatic deposits (white), lymph node metastasis (orange), lymph node metastasis combined with invasion into peritoneum or pelvic muscle (purple), combined lymph node and lung metastasis (brown), animals with both lymph node and lung metastasis combined with invasion into peritoneum or pelvic muscle (black). C, Timing and type of metastatic deposits in Ptenfl/flKrasG12V cohorts. D, Typical epithelial gland-like metastasis in lymph node from Ptenfl/flKrasG12V cohort. Rare sarcomatoid nodules in lymph nodes (E) combined with sarcomatoid metastases in the lung (F) observed in a single mouse (of 20) in the Ptenfl/flKrasG12V cohort scale bar: 200 μm. Heterogeneous lumbar lymph node metastases from Ptenfl/flKrasG12VPLXNB1P1597L mice, including mixed epithelial/sarcomatoid deposits (G), sarcomatoid (H), and squamous metaplasia (I) [scale bar: 200 μm (G–I)]. Organ metastasis and local invasion in Ptenfl/flKrasG12VPLXNB1P1597L mice showing lung metastatic deposit with sarcomatoid (J) and squamous histology (K), abdominal metastasis adjoining pancreas (L) and prostate tumor invading urethra (M). Scale bar: 200 μm (J–L), 500 μm (M). N, The single lymph node deposit observed in the Ptenfl/flKrasG12VPlxnB1WTcohort (scale bar: 200 μm).