Skip to main content
. Author manuscript; available in PMC: 2023 Mar 16.
Published in final edited form as: Methods Mol Biol. 2023;2557:365–390. doi: 10.1007/978-1-0716-2639-9_23

Fig. 1.

Fig. 1

Generation of hTERT RPE1 COG4 KO cell line expressing OsTIR1-9myc and COG4-mAID-mCherry: Cartoon image showing (A) The development of hTERT RPE1 cell line depleted for COG4 subunit by CRISPR-Cas9 approach and retroviral transduction resulting COG4 KO cells to express OsTIR1-9myc. (B) Creation of COG4-mAID stable cell line by rescuing the COG4 KO/OsTIR1-9myc cells by the lentiviral transduction to express COG4-mAID-mCherry under the control of COG4 promoter. (C) The experimental approach for COG4-mAID-mCherry rapid degradation by IAA (auxin) treatment