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. Author manuscript; available in PMC: 2023 Mar 16.
Published in final edited form as: Methods Mol Biol. 2023;2557:365–390. doi: 10.1007/978-1-0716-2639-9_23

Fig. 2.

Fig. 2

Auxin (IAA) treatment induces rapid depletion of hCOG4-mAID-mCherry. (A) The hTERT RPE1 COG4 KO/OsTir1-9myc/hCOG4-mAid-mCherry cells were treated with 0.5 mM IAA for different times as indicated. Cells were collected and lysed, and the expression of wild-type and hybrid COG4 protein was analyzed by WB using an anti-COG4 antibody. (B) Quantification of the COG4 protein level at different time points after IAA treatment. Three independent experiments have been performed. (C) IF analysis of COG4-mAID-mCherry depletion after IAA treatment. The COG4 mAID clone was treated with IAA for 1 h. Untreated cells were used as a control. The cells were fixed and stained for GM130 (green). Scale bars, 20 μm