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. 2023 Mar 16;18(3):e0283244. doi: 10.1371/journal.pone.0283244

Fig 8. Validation of gene expression by qRT-PCR.

Fig 8

HCM-associated differences in expression profiles in the left ventricle (LV: A) and left atrium (LA: B). Comparisons of mRNA levels determined by qRT-PCR show that CXCL14, CXCL6, IL18, THBS4 and ANKRD2 are expressed at higher levels in HCM LV compared to the healthy LV (A), and CXCL14, THBS4, ANKRD2, and ID3 are expressed at higher levels in HCM LA compared to the healthy LA (B). Mean ± SEM (n = 5) values are indicated and the normalized expression values against reference genes RSP7 and GAPDH are plotted.