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. 2023 Mar 10;62(4):53. doi: 10.3892/ijo.2023.5501

Figure 5.

Figure 5

Double-target CAR-T cells display downgraded IR expression, strengthened proliferation capability, and subdued terminal differentiation in long time antigen provoking. (A) Expression levels of PD-1, LAG-3 and TIM-3 in each group at 8, 16 and 24 days in the long-run provoking process. (B) Annexin V+ cell percentage in each group at 8, 16 and 24 days in the long-run provoking process. (C and D) Levels of Bcl-2 and caspase-3 in CAR-T cells on days 8, 16 and 24 in the long-run provoking process examined using reverse transcription-quantitative PCR. (E) Alterations in the total cell number in each group during long-run provoking every 4 days (two-way ANOVA). (F) The expression of CD62L and CD45RA on the CAR-T cells and initial T cells in each group in the long-run provoking process. Data are presented as the mean ± SD. *P<0.05, **P<0.01 and ***P<0.001. ns, not significant. CAR, chimeric antigen receptor; IR, inhibitory receptor; PD-1, programmed death 1; LAG-3, lymphocyte-activation gene 3; TIM-3, T cell immunoglobulin and mucin-domain containing-3.