Figure 3. The impact of mesenchymal stem cells (MSCs) on the expression of key components involved in the formation of NLRP3 inflammasome and mitochondrial reactive oxygen species (ROS).
(A) The protein expression of IL-1β, caspase-1, and AIM2 in cell lysates was analyzed by Western blot. (B) Quantitation of IL-1β secretion in the supernatants by ELISA. (C) FACS analysis of ROS level and mitochondria mass with fluorescent dye CellROX Deep Red and MitoTracker Green. (D) Quantitation of mitochondria-specific ROS level based on the percentage of cells that were both positive for CellROX and MitoTracker. All samples were collected 24 hr post the co-culture of different cells. For the measurements of IL-β, results are shown as the mean ± SD from three independent experiments (p values are as indicated, n=3).
Figure 3—source data 1. Labeled original blots of Figure 3A.
elife-82934-fig3-data1.zip (1.1MB, zip)
Figure 3—source data 2. Unlabeled original blots of Figure 3A.
elife-82934-fig3-data2.zip (912.6KB, zip)
Figure 3—source data 3. Figure 3B in Excel file.
elife-82934-fig3-data3.xlsx (18.3KB, xlsx)
Figure 3—source data 4. Figure 3D in Excel file.
elife-82934-fig3-data4.xlsx (15.5KB, xlsx)