Effects of KXS on the cAMP/PKA/ERK/CREB and Wnt/β-catenin signaling pathways in cells after miR-1281 overexpression. Western blot was used to detect the effects of miR-1281 overexpression on cAMP/PKA/ERK/CREB and Wnt/β-catenin signal transduction pathways. (A–F) The effects of KXS on the expression levels of PKA, pERK1/2, CREB, p-CREB and BDNF proteins in the cAMP signaling pathway in cells after miR-1281 overexpression.(G–J) The effects of KXS on the expression levels of GSK-3β, pGSK-3β and β-catenin proteins in the Wnt signaling pathway in cells after miR-1281 overexpression. The expression of the negative control (NC) group in the experiment was set as 1, and the relative expression levels of the other groups were compared to that of the NC group. Compared with NC, ##P < 0.01; compared with the OE group, *P < 0.05, **P < 0.01.