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. 2023 Mar 17;56(3):526–536. doi: 10.1016/j.jmii.2023.03.003

Figure 1.

Fig. 1

Reactive CD4+ and CD8+ T cells against SARS-CoV-2 proteins. PBMC from 15 LT recipients and 15 controls were stimulated in vitro with proteins M, N, S, and a mixture of M + N + S for 16 h (A) Lymphocytes were gated according to FSC-height and SSC-height characteristics. Then, CD3+CD4+ or CD3+CD8+ T cells were selected, and SARS-CoV-2 reactive T cells were considered as those CD69+CD137+ cells in gated CD3+CD4+ or CD3+CD8+ cells (B) Example plots showing staining patterns of SARS-CoV-2 reactive T cells in gated CD3+CD4+ and CD3+CD8+ T cells in each culture condition (C) Percentage of reactive CD4+ and CD8+ T cells against each SARS-CoV-2 protein in 15 LT recipients and 15 controls. Bars show median percentage and error bars show interquartile range.