Table 3.

Genetic diversity estimates for Rana temporaria populations in the Province of Trento: results of eDNA metabarcoding from the present study compared to the reference dataset⁴¹.

Site	No haplotypes (n)			Haplotype div. (h)			Nucleotide div. (pi)
	eDNA (T1)	eDNA (T1 + T2)	tissue DNA	eDNA (T1)	eDNA (T1 + T2)	DNA	eDNA (T1)	eDNA (T1 + T2)	tissue DNA
Amp	2	2	2	0.368	0.428	0.533	0.001	0.001	9E-04
Bed	3	3	3	0.543	0.545	0.378	0.009	0.01	0.005
Fia	4	4	4	0.644	0.639	0.8	0.002	0.002	0.002
Ing	2	2	2	0.121	0.352	0.351	0.001	0.004	0.003
Lel	2	2	3	0.344	0.344	0.644	0.004	0.004	0.008
PLa	3	3	2	0.349	0.349	0.356	0.009	0.009	0.008
PMa	2	2	2	0.256	0.256	0.467	0.003	0.003	0.004
PS1	3	3	3	0.533	0.509	0.689	0.011	0.01	0.011
PS2	3	3	3	0.575	0.575	0.644	0.013	0.013	0.011
Va1	4	4	4	0.601	0.632	0.778	0.002	0.002	0.002

eDNA (T1): eDNA metabarcoding considering only T1 replicates; eDNA (T1 + T2): eDNA metabarcoding considering T1 + T2 replicates; tissue DNA: estimates from the reference dataset⁴¹.