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. 2023 Mar 16;14:1455. doi: 10.1038/s41467-023-36921-2

Fig. 8. Drug treatment inhibits diacylglycerol to triacylglycerol conversion in trophozoites.

Fig. 8

Young 3D7 trophozoites were enriched based on their magnetic binding to MACS columns and were treated with DMSO, 10 mM MMV665924 or 1 mM MMV897615 for 8 h. Lipids were extracted and submitted for Liquid Chromatography / Mass Spectrometry (LC/MS). Three biological replicates were performed, and results of each sample were normalized to total lipid composition of the sample. Volcano plots showing lipid species for DMSO-treated parasites vs MMV665924 (a) or MMV897615 (b), lipid species that are more than 2-fold different and have a p value <0.05 (two-sided unpaired Student’s t test) are shaded. c Changes in relevant lipid subclasses upon drug treatment compared to DMSO control. d The contribution of each individual FA species to the total TAG pool was analyzed and compared between the DMSO control and the drug-treated parasites. Data are shown for the six most frequently detected FAs. Results were obtained from three biological replicates and are shown as means ± SD. Differences were tested using a two-sided unpaired Student’s t test. a: p = 0.01, b: p = 0.006, c: p = 0.00009, d: p = 0.001, e: p = 0.04, f: p = 0.0002, g: p = 0.0003, h: p = 0.004, i: p = 0.003, j: p = 0.0008, k: p = 0.002, l: p = 0.008. The complete data are available in Supplementary Data 9 and 11, and in the Source Data file. FA fatty acid, TAG triacylglycerol.