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. 2023 Mar 3;10:1045030. doi: 10.3389/fvets.2023.1045030

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Copyright © 2023 Zamith Cunha, Zannoni, Salamanca, De Silva, Rinnovati, Gramenzi, Forni and Chiocchetti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photomicrographs of the cryosections of the synovial membrane of a horse metacarpophalangeal joint showing peroxisome proliferator-activated receptor alpha (PPARα) immunoreactivity in synoviocytes (A–H). (A–C) The white arrows indicate the DAPI (Blue) labeled nuclei of synoviocytes brightly immunolabelled with the anti-vimentin (Red) antibody which expressed faint-to-moderate PPARα (Green) immunoreactivity. It is possible to see the indistinct PPARα immunostaining of the upper portions of the cells lining the joint cavity. (E–H) The figures show the longitudinal sections of two villi of the synovial membrane in which the arrows indicate the DAPI (Blue) labeled nuclei (E) of the cells, likely fibroblast-like synoviocytes and fibroblasts, co-expressing faint-to-moderate PPARα-(Green) (F) and bright vimentin-(Red) (G) immunoreactivity. (D, H) Merged images (Orange). Scale bar = 50 μm.