Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 16;14(3):199. doi: 10.1038/s41419-023-05723-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A–D Mitochondrial Lon interacts with ULK1 shown by co-immunoprecipitation. A HCT-15 cells were treated with CoCl₂ followed by co-immunoprecipitation with anti-ULK1. Whole cell lysates from HCT-15 cells treated with CoCl₂ (200 μM for 18 h) were immunoprecipitated with anti-ULK1 antibodies. The immunoprecipitation complex was analyzed by Western blotting using the indicated antibodies. IP, immunoprecipitation. B Whole cell lysates from HCT-15 cells transfected with the plasmids encoding Flag-ULK1 or vector were immunoprecipitated with anti-ULK1 antibodies. The immunoprecipitation complex was analyzed by Western blotting using the indicated antibodies. C Whole cell lysates from HCT-15 cells transfected with the plasmids encoding myc-Lon and Flag-ULK1 were immunoprecipitated with anti-myc antibodies. The immunoprecipitation complex was analyzed by Western blotting using the indicated antibodies. D Whole cell lysates from HCT-15 cells transfected with the plasmids encoding myc-Lon or myc-LonK529R were immunoprecipitated with anti-myc antibodies. The immunoprecipitation complex was analyzed by Western blotting using the indicated antibodies. E, F Mitochondrial Lon interacts with ULK1 shown by immunofluorescence. E The interaction of Lon with ULK1 was enhanced by ULK1 activity under hypoxia. HCT-15 cells treated with or without CoCl₂ (200 μM for 18 h) in the presence or absence of SBI-0206965 (20 μM for 6 h) were immunostained by anti-ULK1 (green) and anti-Lon (red) following image capturing by immunofluorescence microscopy. DAPI was used for nuclear staining. Scale bars, 50 μm (n = >50 cells/condition and 3 biological replicates). F The interaction of Lon with ULK1 was enhanced by ULK1 activity. HCT-15 cells transfected with the plasmids encoding Lon or empty in the presence or absence of SBI-0206965 (20 μM for 6 h) were immunostained by anti-ULK1 (green) and anti-Lon (red) following image capturing by immunofluorescence microscopy. DAPI was used for nuclear staining. Scale bars, 50 μm (n = >50 cells/condition and 3 biological replicates).