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. 2023 Mar 16;14(3):199. doi: 10.1038/s41419-023-05723-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A HCT-15 cells transfected with the plasmids encoding myc-Lon were used to perform subcellular fractionation experiment. Cell lysates were analyzed by immunoblotting using the indicated antibodies. GAPDH as the loading control. Mito mitochondria, MAM mitochondria associated membranes, ER endoplasmic reticulum, cyto cytosol, PNS post nuclear supernatant. B HCT-15 cells were treated with CoCl₂ (200 μM for 18 h) or not in the presence or absence of SBI-0206965 (20 μM for 6 h). Whole cell lysates from the treated HCT-15 cells were immunoprecipitated with anti-LC3B antibodies. The immunoprecipitation complex was analyzed by Western blotting using the indicated antibodies. C HCT-15 cells transfected with the plasmids encoding Lon or empty vector were treated with SBI-0206965 (20 μM for 6 h) or not. Whole cell lysates from the treated HCT-15 cells were immunoprecipitated with anti-LC3B antibodies. The immunoprecipitation complex was analyzed by Western blotting using the indicated antibodies. D HCT-15 cells were treated with or without CoCl₂ (200 μM for 18 h) or hypoxia exposure (1% O₂). Cell lysates were analyzed by immunoblotting using the indicated antibodies. GAPDH as the loading control. E HCT-15 cells were transfected with the plasmids encoding Lon or Lon-shRNA. Cell lysates were analyzed by immunoblotting using the indicated antibodies. GAPDH as the loading control. F HCT-15 cells transfected with the plasmids encoding Lon or empty were treated with SBI-0206965 (20 μM for 6 h) or not. Cell lysates were analyzed by immunoblotting using the indicated antibodies. GAPDH as the loading control. G HCT-15 cells were transfected with the plasmids encoding myc-Lon, myc-LonK529R (ATPase mutant), or myc-LonS855A (protease mutant). Cell lysates were analyzed by immunoblotting using the indicated antibodies. GAPDH as the loading control. H HCT-15 cells were transfected with the plasmids encoding empty, myc-Lon, or myc-LonK529R in the presence or absence of CoCl₂ treatment (200 μM for 18 h). Cell lysates were analyzed by immunoblotting using the indicated antibodies. GAPDH as the loading control. I Immunohistochemical analysis of p-FUNDC1-S17 expression in OSCC patients. Representative immunohistochemical staining of p-FUNDC1-S17 was performed using paraffin-embedded sections of OSCC. The representative intensity of immunostaining was classified as four levels: negative staining intensity (0) and positive staining intensity, including weak (1+), median (2+), and strong (3+) staining. Microscopic magnification, ×400. Scale bar, 50 μm.