Skip to main content
. 2023 Mar 3;16:1091096. doi: 10.3389/fnmol.2023.1091096

Figure 5.

Figure 5

Activation of Nav1.7+ SDH neurons in SCI mice. (A–L) Double immunostaining for FOS (Red) and Nav1.7 (Green) on spinal section derived from naive (A, D, G, J), sham (B, E, H, K), and SCI (C, F, I, L) mice showed more FOS-positive neurons and more FOS/Nav1.7 double-positive neurons in deep laminae layers of SCI mice. Inset is the high magnification view of the boxed area. Scale bar 100 μm. (M–O) The number of FOS+ (M), Nav1.7+/FOS+ (N), and Nav1.7+ (O) SDH neurons in laminae I–VI of home-caged naive mice, sham, and SCI mice. The symbol # represents the comparison between SCI and sham, and the symbol * represents the comparison between naive and SCI or sham. (P) The ratio of Nav1.7+/FOS+ SDH neurons to total Nav1.7+ SDH neurons in naive (0%), sham (3 ± 4.8%), and SCI (26 ± 8.2%) mice. Data are shown as Mean ± SD, n = 3. # p < 0.05, ## p < 0.01,*p < 0.05, ** p < 0.01, ***p < 0.001, unpaired t-test or one way ANOVA. Scale bar = 100 μm.