Fig. 9.

Effect of ABBSG@EGCG on mitochondria and NF-κb of BMSCs under a diabetic condition.

The mBMSCs were treated with high glucose for 3d before being handled. (A) Representative immunofluorescence of mitochondria, the mitochondrial membrane potential and mitochondrial reactive oxygen species in mBMSCs. Scale bare:10 μm. (B–C) Representative images of western blot and quantitative analyses of p-p65 and p65 (n = 3). (D) Immunofluorescence with NF-κB antibody in mBMSCs under high-glucose (HG; 30 mmol/L) conditions. Scale bare:10 μm. (E) The quantify of mitochondrial morphology in mBMSCs (n = 6). (F) TGF-β1 concentration in mBMSCs, as determined by ELISA (n = 3). (G) Proliferation of mBMSCs after HG incubation for 1, 3 and 5 days. Quantified by cck8 kit. All results are representative of the data generated in at least three independent experiments and are presented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA.