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. 2023 Mar 3;10:1097609. doi: 10.3389/fmolb.2023.1097609

FIGURE 3.

FIGURE 3

Region III of Spot 42 is involved in mRNA degradation of the 3′ end-sharing mRNA only. (A) Nucleotide sequence of gal, Spot 42, and Spot 42 mutants. The Shine and Dalgarno sequence for translation initiation of galK is presented in red. The translation stop codon of galT and the translation initiation codon of galK are presented in blue and green, respectively. Also, about 50 nucleotide sequences of the 5′ portion of Spot 42 are presented in red below the gal sequence. The three regions of Spot 42 that form perfect base pairings with the gal nucleotide sequences are identified as regions I, II, and III. Black dots represent base pairing. Spot 42 mutations in each region are listed under the corresponding region by name. Nucleotide changes are marked in red. The region I mutations are nucleotide deletions denoted as Δ in red. (B) K-probed Northern blot of the region I mutant; (C) K-probed Northern blot of the region II mutant; (D) K-probed Northern blot of the region III mutants; (E) E-probed Northern blot of the region III mutants.