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. 2023 Mar 3;10:1097609. doi: 10.3389/fmolb.2023.1097609

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Copyright © 2023 Jeon, Lee, N, Kang and Lim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Spot 42 negatively regulates RNase E cleavage that generates galKM. Nucleotide sequences upstream of the translation initiation codons of galT (A), galK (B), and galM (C), show the correspondence of the cleavage positions (inverted arrows) of the RNase E consensus cleavage sites (thick underlines). Also highlighted are the putative Shine–Dalgarno sequences (in bold red) and the initiation (green) and termination (blue) codons. The RNase E cleavages in (A), (B), and (C) result in the generation of the 5′ end of galTKM, galKM, and galM, respectively. Base pairing of region III to the corresponding regions of gal are presented with dots. (D) 5′RACE results show the 5′ ends of galTKM at 1,031 and 1,039 in wild type (WT) (lane 1) and Δspf (lane 2), the 5′ ends of galKM at 2,071 and 2,079 in wild type (WT) (lane 3), and Δspf (lane 4), and the 5′ ends of galM at 3,209, 3,211, and 3,217 in wild type (WT) (lane 5) and Δspf (lane 6).