TABLE 1.
Primers were used for amplification of virulence and β‐lactamase genes.
| Gene | Primer sequence | Amplicon size (bp) | Reference |
|---|---|---|---|
| exoA |
F: GACAACGCCCTCAGCATCACCAGC R: CGCTGGCCCATTCGCTCCAGCGCT |
396 | 19 |
| nan1 |
F: ATG AAT ACT TAT TTT GAT AT R: CTA AAT CCA TGC TCT GAC CC |
1316 | 20 |
| lasB |
F: AGCCATCACCGAAGTCAAGG R: CGGGAATCAGGTAGGAGACG |
250 | 21 |
| ExoS |
F: CTT GAA GGG ACT CGA CAA GG R: TTC AGG TCC GCG TAG TGA AT |
504 | 21 |
| algD |
F: ATG CGA ATC AGC ATC TTT GGT R: CTA CCA GCA GAT GCC CTC GGC |
1310 | 21 |
| plcH |
F: GAA GCC ATG GGC TACTTCAA R: AGA GTG ACG AGG AGC GGTAG |
307 | 21 |
| bla SHV |
F: GCCCGGGTTATTCTTATTTGTCGC R: TCTTTCCGATGCCGCCGCCAGTCA |
1013 | 22 |
| bla TEM |
F: TCCGCTCATGAGACAATAACC R: ATAATACCGCACCACATAGCAG |
300 | 22 |
| bla CTX‐M |
F: TTTGCGATGTGCAGTACCAGTAA R: CGATATCGTTGGTGGTGCCATA |
455 | 22 |
| bla VIM |
F: GATGGTGTTTGGTCGCATA R: CGAATGCGCAGCACCAG |
390 | 22 |
| bla IMP |
F: AGCCCATAGTTAACCCCGCC R: CTGGCTTAATTCTCAATCCATCCC |
114 | 22 |
| bla NDM |
F: GGTTTGGCGATCTGGTTTTC R: CGGAATGGCTCATCACGATC |
621 | 22 |
| bla oxa‐23 |
F: TGGAAGGGCGAGAAAAGGTC R: TTGCCCAACCAGTCTTTCCA |
400 | 22 |
| bla oxa‐48 |
F: GCGTGGTTAAGGATGAACAC R: CATCAAGTTCAACCCAACCG |
438 | 22 |
| bla oxa‐11 |
F: CGAGTACGGCATTAGCTGGT R: CTCTTGGCTTTCCGTCCCAT |
250 | 22 |