Fig. 7.

Behavioral defects in CK-p25TgOE mice rescued after TFP5 treatment. Various CK-p25 cohorts were tested for behavior assays including Y maze, Rota-rod and Open field. A) Spatial memory deficit was measured by Y maze assay represented in the bar graph as average % alternation. The bi-transgenic CK-p25Tg performed poorly (less than 50%) and the poor performance was rescued in the CK-p25TgOE mice injected with TFP5. Scrambled peptide had no rescue affect. B) Motor deficits were accessed via rota-rod experiments. The average time (in seconds, as shown in the bar graph) for TFP5 injected CK-p25TgOE mice was comparable to the control cohorts, whereas PBS or scrambled injected mice could not balance themselves well on the rotating rod. C-D). Open-field test was carried out to observe the exploratory (total distance travelled over 20 min time) and anxiety behavior (time spent at the center of the box). Even though there was a significant decrease in the total distance travelled in the open field by TFP5 injected CK-p25TgOE mice compared with PBS or scrambled injected mice, there was no significant difference in the time spent at center of the open field box between the cohorts. Each column in the graph represents the mean ± SEM. (n = 10–16 and *p ≤ 0.05, **p ≤ 0.01).