Table 7.

List of antimicrobial compounds retrieved from LCMS analysis of T. harzianum MC2 and T. harzianum NBG.

A. Significant metabolites derived from LCMS extract of T. harzianum NBG
Sl.no	Molecule	Formula	Match score	Similarity	Retention time	Molecule Class	Function	Reference
1	Trichodin A	C21H25NO3	0.982	0.982	7.87	Pyridine	Antibacterial, Antifungal	(Wu et al., 2014)
2	Viridiol	C20H18O6	0.986	0.986	7.87	Steroid	Antifungal	(Moffatt et al., 1969)
3	5-hydroxy-2,3-dimethyl-7-methoxychromone	C12H12O4	0.88	0.88	15.76	Ketone	No obvious function
4	Alternariol monomethyl ether	C15H12O5	0.879	0.879	16.52	Ether	Mycotoxin	(Demuner et al., 2013)
5	(9R,10R)-dihydro-harzianone	C20H32O	0.914	0.914	17.73	Diterpene	Cytotoxic	(Ortega et al., 2021)
6	Cyclonerodiol oxide	C15H28O3	0.907	0.907	17.73	Sesquiterpene	Plant growth regulation	(Cutler et al., 1991)
7	5-hydroxyvertinolide	C14H18O5	0.967	0.967	17.76	Butenolide	Antifungal	(Vinale et al., 2009b)
8	Trichocarotin C	C15H22O3	0.955	0.955	17.76	Sesquiterpene	Antimicroalgal	(Shi et al., 2018)
9	Citrinoviric acid	C14H20O6	0.966	0.966	19.69	Polyketides	Cytotoxic	(Zhang et al., 2014)
10	Fleephilone	C24H27NO7	0.876	0.876	21.72	Azaphilones	No obvious activity	(Qian-Cutrone et al., 1996)
11	Tenuazonic acid	C10H15NO3	0.95	0.95	23.1	Tetrameric acid	Phytotoxin	(Yun et al., 2015)
12	Pyridoxatin	C15H21NO3	0.949	0.949	25.14	Dihydroxypyridines	Antifungal, Antibiotic	(Wu et al., 2014)
13	Penicillenol D/Coprogen	C17H29NO4	0.88	0.88	30.06	Hydroxamate siderophores	Cytotoxic	(Anke et al., 1991)
14	Isoechinulin A	C24H29N3O2	0.948	0.948	33.99	Alkaloids	Antialgal	(Shi, 2018)
15	Ergosterol	C28H44O	0.875	0.875	33.99	Sterol	Antifungal	(Tarus et al., 2003)
B. Significant metabolites derived from LCMS extract of T. harzianum MC2
Sl.no	Molecule	Formula	Match score	Similarity	Retention time	Molecule class	Function	References
1	5-Hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone	C12H12O5	0.995	0.995	14.07	Ketone	No obvious activity
2	3-hydroxytrichodenone C	C7H9ClO3	0.946	0.946	16.1	Ketone	Antibacterial, Growth Inhibiting	(Song et al., 2018)
3	Flaviolin	C10H6O5	0.916	0.916	16.1	Naphthoquinone	No obvious activity	(Astill and Roberts, 1953)
4	Cyclonerodiol oxide	C15H28O3	0.88	0.88	17.69	Sesquiterpene	Plant growth regulation	(Cutler et al., 1991)
5	Viridin	C20H16O6	0.873	0.873	19.93	Steroidal antibiotics	Antifungal	(Brian and McGowan, 1945)
6	Trichoderone B	C24H35NO5	0.938	0.938	20.65	Cytochalasans	Cytotoxic	(Ding et al., 2012)
7	Cyclopenol	C17H14N2O4	0.914	0.914	21.86	Alkaloid	Antibacterial	(Shi, 2018)
8	Gliotoxin	C13H14N2O4S2	0.908	0.908	21.86	Diketopiperazines	Antifungal	(Singh et al., 2005)
9	Cremenolide	C16H22O7	0.998	0.998	21.86	Lactone	Antifungal and PGP	(Vinale et al., 2016)
10	(9R,10R)-dihydro-harzianone	C20H32O	0.899	0.899	21.9	Diterpenes	Cytotoxic	(Ortega et al., 2021)
11	Trichodermaerin	C20H28O3	0.943	0.943	21.9	Diterpenes	No obvious activity	(Chantrapromma et al., 2014)
12	Citrinoviric acid	C14H20O6	0.977	0.977	25.03	Polyketides	Cytotoxic	(Zhang et al., 2014)
13	Pyridoxatin	C15H21NO3	0.968	0.968	25.07	Dihydroxypyridines	Antibiotic	(Wu et al., 2014)
14	Harzianic acid	C19H27NO6	0.917	0.917	27.51	Tetramic acid derivatives	PGP	(Vinale et al., 2009a)
15	Scytalone	C10H10O4	0.99	0.99	29.86	Lyases	No obvious activity	(Zhang et al., 2017)
16	Ferulic acid	C10H10O4	0.99	0.99	29.86	Hydroxycinnamic acids/phenol	No obvious activity	(Yu et al., 2002)
17	Isoechinulin A	C24H29N3O2	0.928	0.928	33.86	Alkaloid	Antimicroalgal	(Shi, 2018)

*Ferulic acid, Scytalone, Harzianic acid, Cremenolide, viridian, 3-hydroxytrichodenone C.