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Dot blot analysis of Aβ oligomers prepared in the presence or absence of Aβ8-20. Samples were spotted after 48 h incubation at 4 °C under gentle rotation. Membranes were blotted with the following antibodies: anti-Aβ N-terminal 1–16 mouse monoclonal antibody 6E10 (1:100); anti-Aβ 17–24 mouse monoclonal antibody 4G8 (1:100); or anti-oligomer A11 rabbit polyclonal antibody (1:100).
