Figure 3.

Combined inhibition of both GSK3 paralogs is necessary for nuclear translocation of β-catenin in SH-SY5Y cells. To examine β-catenin activation, we quantified translocation of this protein to the nucleus in SH-SY5Y cells 6 h after compound addition at a 9-point dose response curve (range: 20 nM–20 μM). (A–C) EC₅₀ curves demonstrating concentration of GSK3 inhibitors AZ1080 (A), BRD0705 (B) and compound 1 (C) necessary to trigger β-catenin translocation to the nucleus. Each data point represents the mean ± SEM from four biological replicates within a single run of the assay. (D) Representative images showing DAPI nuclear stain in blue and β-catenin in green for each GSK3 inhibitor compound at the highest dose tested (20 μM).