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. Author manuscript; available in PMC: 2023 Mar 17.
Published in final edited form as: Life Sci. 2021 Oct 31;287:120114. doi: 10.1016/j.lfs.2021.120114

Figure 5. Inhibition of cMYC and HIF1α pathways can diminish TLR7-instigated inflammatory and glycolytic regulators in RA FLS.

Figure 5.

RA FLS were untreated (PBS) or pretreated with cMYCi (50μM) or HIFi (2μM) o/n before R837 (1μg/ml) stimulation for 6h. Thereafter transcriptional regulation of monokines (A), transcriptional factors (B), metabolic intermediates (C) or oxidative enzymes (G) were determined by realtime RT-PCR, n=4. Conditioned media were obtained from RA FLS untreated (PBS) or pretreated with cMYCi (50μM) or HIFi (2μM) before R837 (1μg/ml) activation for 24h and measuring of Lactate (D) Citrate (E) and Succinate (F) levels by colorimetric assay, n=4. The data are shown as mean ± SEM, * represents p<0.05 and ** denotes p<0.01.