Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 17;30(7):973–984. doi: 10.1038/s41417-023-00604-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A The binding capacity was measured at the indicated concentration using an ELISA-based assay. Similar results were obtained in three independent experiments. B SW480 cells were pretreated with 2.5 µM of divalent peptide3A5, and then stimulated with human S100A8 recombinant protein. After 30 min, cell lysates were subjected to western blotting using the indicated antibody. The divalent peptide#3 or peptide3A5 reduced the expression of S100A8-mediated IL-8 (C) and VEGF (D) induction. Total RNAs from SW480 cells with/without treatment of the peptide candidates were extracted and qPCR analysis was performed using relevant primer sets and SYBR green. Relative expression levels were compared. Peptide #3 suppresses S100A8-mediated IL-8(E) and VEGF (F) secretion in SW480 cells. Data are shown as mean ± SD. *P < 0.05 compared with treatment of S100A8.