FIG 15.

Effect of HCMV infection on Pol II protein isoforms. Kasumi-3 cells were infected with TB40/Ewt-GFP, and at 1 dpi, GFP-expressing cells were purified by flow cytometry. (A) Lysates were used for Western blot (WB) analysis against Rpb1, phospho (p)-Ser2 Rpb1, and p-Ser5 Rpb1. Cyclophilin B was used as a loading control (B). Quantification of protein expression levels from three independent experiments. Error bars represent standard errors of the means (SEMs). P value <0.05 by one-tailed Student’s pairwise t test.