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. 2023 Mar 17;23:146. doi: 10.1186/s12870-023-04112-2

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Silencing of the NbWAK/WAKLs in Nicotiana benthamiana makes it susceptible to infection with tomato yellow leaf curl virus (TYLCV). a Silencing efficiency was assessed by quantitative PCR (qPCR) at ten days post-inoculation (dpi). The values represent relative mRNA levels against those of the control groups (N. benthamiana seedlings agroinfiltrated with TRV:GFP), values of which are set to 100%. b Disease symptoms caused by TYLCV in the NbWAK/WAKLs silenced N. benthamiana seedlings at 21 dpi. TRV:GFP-agroinfiltrated N. benthamiana seedlings infected with TYLCV were used as the control. c Relative accumulation of TYLCV genomic DNA in the NbWAK/WAKLs silenced N. benthamiana seedlings. Viral accumulation was measured by qPCR at 21 dpi, as shown in Fig. 7a. The values represent relative viral DNA accumulation levels against those of the control groups (TRV:GFP-agroinfiltrated N. benthamiana seedlings infected with TYLCV), values of which are set to 1.0 units. For a and c, the data are given as means ± standard deviation of three biological replicates. Significant differences in expression are marked with asterisks: * P < 0.05 or ** P < 0.01; Student’s t-test