Table 1.
Structural and biochemical features of the WAK/WAKL gene family in N. benthamiana
| Name | Gene IDa | Gene position | Strand | Size | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Genomic DNA (bp) | mRNA (bp) | CDSb (bp) | 5′-UTRc (bp) | 5′-UTRd (bp) | Protein (aa) | pIe | MWf (kDa) | ||||
| NbWAK1 | Niben101Scf00149g10001 | Niben101Scf00149: 1014401..1025094 | forward | 10,694 | 2912 | 2811 | 101 | 0 | 936 | 8.0 | 103.7 |
| NbWAK2 | Niben101Scf00149g10003 | Niben101Scf00149: 1039140..1052317 | reverse | 13,178 | 1707 | 1707 | 0 | 0 | 568 | 6.7 | 62.0 |
| NbWAK3 | Niben101Scf00530g11020 | Niben101Scf00530: 1143477..1146128 | reverse | 2652 | 2075 | 1917 | 42 | 116 | 638 | 7.2 | 72.2 |
| NbWAK4 | Niben101Scf01237g11009 | Niben101Scf01237: 1118347..1122027 | forward | 3681 | 2205 | 2205 | 0 | 0 | 734 | 6.0 | 81.6 |
| NbWAK5 | Niben101Scf02160g02006 | Niben101Scf02160: 217154..220237 | reverse | 3084 | 2707 | 1905 | 291 | 511 | 634 | 6.4 | 71.4 |
| NbWAK6 | Niben101Scf02608g01007 | Niben101Scf02608: 112748..119830 | forward | 7083 | 2385 | 2115 | 270 | 0 | 704 | 6.8 | 77.6 |
| NbWAK7 | Niben101Scf02608g01008 | Niben101Scf02608: 148832..157171 | forward | 8340 | 3204 | 2115 | 270 | 819 | 704 | 6.6 | 77.6 |
| NbWAK8 | Niben101Scf03202g03004 | Niben101Scf03202: 362629..366940 | forward | 4312 | 2247 | 2247 | 0 | 0 | 748 | 6.1 | 82.6 |
| NbWAK9 | Niben101Scf03202g04015 | Niben101Scf03202: 391694..396324 | forward | 4631 | 2202 | 2202 | 0 | 0 | 733 | 7.7 | 79.9 |
| NbWAK10 | Niben101Scf03202g04017 | Niben101Scf03202: 397065..401394 | reverse | 4330 | 2157 | 2157 | 0 | 0 | 718 | 6.4 | 79.7 |
| NbWAK11 | Niben101Scf03472g00005 | Niben101Scf03472: 41760..50789 | reverse | 9030 | 1953 | 1953 | 0 | 0 | 650 | 8.5 | 72.9 |
| NbWAK12 | Niben101Scf06394g08019 | Niben101Scf06394: 877593..890273 | forward | 12,681 | 3083 | 2868 | 215 | 0 | 955 | 6.0 | 105.4 |
| NbWAK13 | Niben101Scf10330g02004 | Niben101Scf10330: 231600..234369 | reverse | 2770 | 2206 | 1893 | 107 | 206 | 630 | 5.7 | 68.3 |
| NbWAK14 | Niben101Scf13018g00012 | Niben101Scf13018: 12405..25275 | reverse | 12,871 | 3699 | 3480 | 219 | 0 | 1159 | 5.9 | 128.2 |
| NbWAK15 | Niben101Scf21196g00008 | Niben101Scf21196: 20786..24874 | forward | 4089 | 1854 | 1854 | 0 | 0 | 617 | 8.3 | 69.0 |
| NbWAKL1 | Niben101Scf00149g10020 | Niben101Scf00149:1031476..1037918 | forward | 6443 | 3469 | 1203 | 88 | 2178 | 400 | 5.1 | 42.9 |
| NbWAKL2 | Niben101Scf00327g01037 | Niben101Scf00327:124864..127845 | reverse | 2982 | 2291 | 1941 | 122 | 228 | 646 | 8.5 | 72.5 |
| NbWAKL3 | Niben101Scf00530g11014 | Niben101Scf00530:1137409..1142320 | reverse | 4912 | 3686 | 1509 | 1733 | 444 | 502 | 5.9 | 57.0 |
| NbWAKL4 | Niben101Scf00700g06021 | Niben101Scf00700:668335..673932 | reverse | 5598 | 2661 | 2661 | 0 | 0 | 886 | 6.2 | 98.8 |
| NbWAKL5 | Niben101Scf01521g06001 | Niben101Scf01521:684767..686339 | reverse | 1573 | 1476 | 915 | 194 | 367 | 304 | 4.9 | 33.6 |
| NbWAKL6 | Niben101Scf02290g02006 | Niben101Scf02290:197165..201565 | forward | 4401 | 2052 | 1908 | 133 | 11 | 635 | 8.4 | 71.1 |
| NbWAKL7 | Niben101Scf02381g08016 | Niben101Scf02381:792605..794693 | reverse | 2089 | 1023 | 1023 | 0 | 0 | 340 | 6.1 | 38.1 |
| NbWAKL8 | Niben101Scf03202g03002 | Niben101Scf03202:301027..307217 | forward | 6191 | 1011 | 1011 | 0 | 0 | 336 | 9.4 | 37.7 |
| NbWAKL9 | Niben101Scf03304g01026 | Niben101Scf03304:167027..172041 | reverse | 5015 | 2064 | 2064 | 0 | 0 | 687 | 6.2 | 76.2 |
| NbWAKL10 | Niben101Scf03363g01019 | Niben101Scf03363:210073..212041 | reverse | 1969 | 1807 | 1029 | 90 | 688 | 342 | 8.3 | 39.0 |
| NbWAKL11 | Niben101Scf03445g00009 | Niben101Scf03445:15851..17440 | forward | 1590 | 1176 | 843 | 333 | 0 | 280 | 5.5 | 30.5 |
| NbWAKL12 | Niben101Scf03939g06023 | Niben101Scf03939:737517..742076 | forward | 4560 | 1395 | 1128 | 0 | 267 | 375 | 6.1 | 41.5 |
| NbWAKL13 | Niben101Scf04445g01002 | Niben101Scf04445:135918..147686 | reverse | 11,769 | 2886 | 2886 | 0 | 0 | 961 | 8.5 | 107.5 |
| NbWAKL14 | Niben101Scf05368g07009 | Niben101Scf05368:738506..740861 | reverse | 2356 | 1865 | 1584 | 0 | 281 | 527 | 6.3 | 59.0 |
| NbWAKL15 | Niben101Scf06394g08020 | Niben101Scf06394:862041..863966 | forward | 1926 | 789 | 789 | 0 | 0 | 262 | 5.1 | 27.8 |
| NbWAKL16 | Niben101Scf06909g04005 | Niben101Scf06909:411031..419054 | reverse | 8024 | 1899 | 1899 | 0 | 0 | 632 | 5.1 | 71.1 |
| NbWAKL17 | Niben101Scf07969g00010 | Niben101Scf07969:42176..44432 | forward | 2257 | 1066 | 888 | 178 | 0 | 295 | 5.3 | 32.5 |
| NbWAKL18 | Niben101Scf11389g01034 | Niben101Scf11389:144908..147896 | forward | 2989 | 1529 | 951 | 197 | 381 | 316 | 4.9 | 34.9 |
| NbWAKL19 | Niben101Scf11416g00017 | Niben101Scf11416:76861..79712 | reverse | 2852 | 1899 | 1899 | 0 | 0 | 632 | 5.9 | 70.2 |
| NbWAKL20 | Niben101Scf14950g00001 | Niben101Scf14950:25106..26461 | reverse | 1356 | 1040 | 864 | 176 | 0 | 287 | 5.3 | 31.4 |
| NbWAKL21 | Niben101Scf20037g00022 | Niben101Scf20037:58700..62033 | reverse | 3334 | 2613 | 2103 | 168 | 342 | 700 | 7.9 | 77.1 |
| NbWAKL22 | Niben101Scf21589g00004 | Niben101Scf21589:55217..57416 | reverse | 2200 | 1074 | 858 | 216 | 0 | 285 | 6.1 | 31.1 |
| NbWAKL23 | Niben101Ctg15342g00002 | Niben101Ctg15342:201..1505 | forward | 1305 | 862 | 609 | 253 | 0 | 202 | 5.3 | 22.3 |
a Gene ID, the gene locus in the Sol Genomics Network (https://solgenomics.net/). b CDS coding sequence. c 5′-UTR 5′-untranslated region. d 3′-UTR 3′-untranslated region. e pI, isoelectric point. f MW, molecular weight