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. 2023 Mar 17;16:23. doi: 10.1186/s13045-023-01413-9

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Combined depletion of ITGB4 and E-/P-selectin cooperatively reduces human ovarian cancer xenograft tumor formation. shRNA-mediated knockdown of ITGB4 in SKOV3 cells, survival of mice after i. p. injection of shControl vs. shITGB4 cells into E-/P-selectin wildtype vs. knockout SCID mice (endpoint: palpable abdominal tumor masses or ascites) and representative anti-ITGB4 immunostaining images from i. p. SKOV3 xenograft tumors (A). Intraperitoneal carcinosis score (ICS) determined as illustrated and spontaneous pulmonary metastatic cell load determined by Alu-PCR on day 57 after i. p. injection of SKOV3 shControl vs. shITGB4 cells into E-/P-selectin wildtype vs. knockout SCID mice (B). Representative anti-ITGB4 immunostaining images of spontaneous SKOV3 lung metastases (arrowhead indicates an ITGB4⁻ lung metastasis) (B). All statistical comparisons indicated by asterisks were calculated vs. shControl/ WT. ***p < 0.001