Fig. 9.

Combined depletion of ITGB4 and E-/P-selectin synergistically reduces pancreatic cancer growth in fully immunocompetent mice. mITGB4 knockdown status in Panc02 murine pancreatic cancer cells prior injection (A). Tumor weight at necropsy and growth periods after s. c. injection of Panc02 control vs. ITGB4 knockdown cells into E-/P-selectin wildtype vs. knockout C57BL/6 mice (B). Representative anti-mITGB4 immunostaining images of Panc02 tumor tissues (C). Quantification of murine CD3⁺ and CD8⁺ lymphocytes per viewing field (VF) in the center and at the margin of s.c. Panc02 tumors across the different groups and representative anti-mCD3 immunostaining images from the center and margin of a Panc02 tumor. Black lines in scatter plots represent mean values. **p < 0.01