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. 2023 Mar 17;42:65. doi: 10.1186/s13046-023-02638-9

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — METTL3 induces SCLC chemoresistance in vitro. A-B CCK-8 assays showed that knockdown of METTL3 decreased the chemotherapy IC50 values in chemoresistant H69AR and H446DDP cells. *P < 0.05, **P < 0.01. C-D Cell apoptosis and arrest were evaluated by flow cytometric analysis in METTL3-knockdown SCLC cells after treatment with chemotherapeutic drugs. E Apoptosis-related protein levels were measured by Western blotting following treatment with chemotherapeutic drugs in METTL3 knockdown cells. F-G CCK-8 assays showed that METTL3 overexpression increased the IC50 values of chemotherapeutic agents in chemosensitive H69 and H446 cells. *P < 0.05, **P < 0.01. H-I Cell apoptosis and arrest were evaluated by flow cytometry in METTL3-overexpressing SCLC cells following treatment with chemotherapeutic drugs. J Apoptosis-related proteins were measured by Western blotting following treatment with chemotherapeutic drugs in METTL3-overexpressing cells. K Western blot assays were performed to evaluate METTL3 expression after transfection with pcDNA3.1-METTL3 and pcDNA3.1-METTL3-mut in H69 cells. L CCK-8 assay showed that overexpression of METTL3 changed the IC50 value of chemotherapy drugs in SCLC cells. **P < 0.01; ns, not significant