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. 2023 Jan 7;191(3):1771–1788. doi: 10.1093/plphys/kiad007

Figure 3.

Figure 3

MdSIZ1 reduces leaf cuticle permeability. A, Water droplets attached to the apple leaves. Leaves sampled from the same location of apple seedlings. B, The contact area between the water droplets and the leaf surface. Error bars indicate standard deviation (Sd; n = 10). Different letters indicate significant differences (ANOVA, Student's t test; P < 0.05). C, Toluidine blue (TB) staining (0.5% [v/v] solution) of apple leaves. White arrows indicate the cuticle stained blue with TB. Scale bar, 1 cm. D, TB uptake was quantified in MdSIZ1 transgenic and WT plants in (C) using a spectrophotometer with three biological replicates. Error bars indicate standard deviation (Sd; n = 3). Different letters indicate significant differences (ANOVA, Student's t test; P < 0.05). E, Water loss rate of apple leaves. The decrease in weight every 10 min at room temperature corresponds to the decrease in moisture. Error bars indicate standard deviation (Sd; n = 3) (F) Chlorophyll leaching assays of apple leaves. Chlorophyll μmol/mg FW (fresh weight, FW). Error bars indicate standard deviation (Sd; n = 3).