Skip to main content
. 2022 Dec 19;191(3):1953–1967. doi: 10.1093/plphys/kiac586

Figure 2.

Figure 2

ABA promotes auxin accumulation to inhibit root growth. A, Representative images of DR5-GUS expression in root with or without ABA treatment. Seedlings of 4-d-old transgenic lines containing DR5-GUS were treated with or without 1 μM ABA for 12 h before GUS activity was assayed. Bar = 1 cm. B, Representative longitudinal sections of meristematic zone (MZ), elongation zone (EZ) and differentiation zone (DZ) in panel (A). Bar = 100 μm. C, Expression of YUCs, TAA1 and TAR1 in the roots of 4-d-old wild-type seedlings treated with or without 1 μM ABA for 4 h. The data are shown as mean ± SD; n = 3 biological replicates. D, Indole-3-acetic acid (IAA) contents in 4-d-old wild-type seedlings treated with or without 1 μM ABA for 24 h. The data are shown as mean ± SD; n = 3 biological replicates. (E and F) Morphology (E) and primary root length (F) of 4-d-old wild-type seedlings grown in the absence or presence of 0.5 μM ABA, with or without supplementation of 5 μM yucasin or L-Kyn. Bar = 1 cm. Data are means ± SD (n ≥ 30 independent seedlings). The individual images in (E) were digitally extracted for comparison. For (C), (D) and (F), asterisks indicate significant differences compared with mock at *P < 0.05 and **P < 0.01 (Student's t-test).