Fig. 1.

Drift correction of 3D videos using Fast4DReg. Scheme highlighting the Fast4DReg pipeline. (A) Fast4DReg sequentially estimates the lateral drift, corrects the lateral drift, and then estimates and corrects the axial drift. Fast4DReg creates intensity projections along multiple axes and estimates the drift between the reference and moving frames by calculating their cross-correlation matrix (CCM). The location of the peak intensity in the CCM (pink asterisk) defines the linear shift between the two images (as highlighted by the pink double-headed arrow). Fast4DReg outputs the corrected images, the drift plots, a drift table, and a settings file containing all selected parameters and paths to the drift table. (B) The settings file inducing the used parameters and path to the drift table can then be applied to correct other datasets (i.e. another channel) directly.