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Trypan blue dye exclusion assay. Percent (%) dead cells in HCT-116 cells treated with different doses of (A) M1 and (B) M2 (100–5,000 μg/mL) for 24 h. The results shown are the mean ± SEM of three independent experiments performed in triplicate (ns > 0.01, *p < 0.01, **p < 0.001, and ***p < 0.0001 represent significant difference compared with control).
