Fig. 1.

CCDC86 is a nucleolar protein associated with the chromosome periphery. (A,B) Two-dimensional scatter plot showing the fold-change (log10) of protein abundance on mitotic chromosomes (cI, A) or in vitro exchange on chromosomes (cIII, B) versus enrichment on chromosomes (cII) generated in previous proteomics analysis (Ohta et al., 2010). The colours indicate the different chromosome periphery proteins as indicated in the legend. The colour intensities, ruled by the smooth density plot in R, indicate the population densities. (C) CCDC86 interactome (GPS-Prot – human protein–protein interactions). Known nucleolar proteins are highlighted in red and histones in yellow. Interacting proteins are coloured green. (D) A GFP–CCDC86 construct (green) was transfected in HeLa cells. Cells were fixed and stained for Ki-67 (red) and DNA (blue). Images representative of >10 independent experiments showing GFP–CCDC86 localisation in interphase (top row), metaphase (middle row) and telophase (bottom row). Scale bar: 5 µm.