Table 1. HG culture conditions do not impact BeWo trophoblast glycolytic activity.
Differentiation state |
Treatment | Basal glycolytic rate (ECAR/μg DNA) |
Max glycolytic rate (ECAR/μg DNA) |
Reserve glycolytic capacity (% basal glycolysis) |
Non-glycolytic acidification (ECAR/μg DNA) |
---|---|---|---|---|---|
CT | LG | 75.71 ± 15.38 | 101.4 ± 20.92 | 133.8 ± 1.14 | 19.26 ± 4.43 |
HG | 67.54 ± 7.30 | 88.23 ± 11.82 | 129.5 ± 4.08 | 18.38 ± 3.10 | |
SCT | LG | 69.21 ± 12.67 | 84.45 ± 14.55 | 123.5 ± 3.70 | 20.50 ± 3.39 |
HG | 77.38 ± 6.78 | 88.01 ± 8.17 | 113.6 ± 1.45 | 23.30 ± 2.45 | |
*Differentiation state difference | NS | NS | * | NS |
Data are mean ± SEM (n = 5/group). Differences in mitochondrial respiratory activity parameters between observed BeWo CT and SCT differentiation states found in the two-way ANOVA test are denoted as
* p<0.05.