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. 2023 Mar 17;12:e83760. doi: 10.7554/eLife.83760

Figure 6. Ntn1 cKO in DATIRES::Vgat-Cre mice partially rescues behavioral phenotype.

(A) Model of Ntn1 loss of function in the ventral tegmental area (VTA) on excitatory and inhibitory balance. (B) Schematic of GABA and Dopamine Ntn1 cKO. (C) Distance traveled in 15 min bins over the course of three nights and two days (Two-Way ANOVA Group F(1, 45)=0.004273, p>0.05, Time F(17.16, 772.1)=23.36, p<0.0001, Interaction F (247, 11115)=1.492, p<0.0001). (D) Earned reinforcers during three days of FR1 or FR5 operant conditioning (n=21 controls, n=20 Ntn1 cKO, FR1: Group F(1, 40)=0.04247 p=0.8378, Time F(2, 80)=25.70 p<0.0001, Interaction F(2, 80)=1.402 p=0.2522; FR5: Group F(1, 40)=0.2244 p=0.6383, Time F(1.499, 59.95)=2.226 Pp0.1295, Interaction F(2, 80)=0.1385 p=0.8708) (E) Breakpoint (maximum presses per reinforcer) on a progressive ratio task (t=2.502, df = 39, *p<0.05) (F) Lever presses per session during five days of extinction training (Group F(1, 39)=6.990, *p=0.0117, Time F(2.381, 92.87)=42.95 p<0.0001, Interaction F(4, 156)=0.1470 p=0.9641). (G) Acoustic startle response to varying intensity white noise stimuli (Group F(1, 40)=0.1207 p=0.7301, Intensity F(1.775, 70.99)=36.77 p<0.0001, Interaction F(6, 240)=0.6127 p=0.7201). (G) Percent inhibition of startle response following pre-pulse at indicated intensities (Group F(1, 120)=0.9661 p=0.3276, Intensity F(2, 120)=7.067 p=0.0013, Interaction F(2, 120)=0.8861 p=0.4150). (H) Time on edge or in center of open field arena during a 10 min test session (edge: t=0.3584, df = 45 p>0,05, center: t=0.4233, df = 45, p>0.05).

Figure 6—source data 1. Behavioral data for Figure 6.
elife-83760-fig6-data1.xlsx (109.2KB, xlsx)

Figure 6.

Figure 6—figure supplement 1. Average day and night locomotion in DAT-Cre::Vgat-Cre mice.

Figure 6—figure supplement 1.

(A) Ambulatory activity (beam breaks) averaged across three nights and two days (n=26 controls n=21 cKO, Night: t=1.010, df = 45, p=0.3178 and Day: t=2.091, df = 45 *p<0.05). (B) Distance traveled during open field test (t=1.414, df = 45, p>0.05). (C) Initial mouse weight prior to instrumental conditioning (n=10 control, n=13 cKO, t=0.08465, df = 21 p>0.05). (D) FR1 reinstatement (t=1.127, df = 39 p>0.05) (E) Extinction Day 4 lever presses per 10 min time bins (Two-way ANOVA Group F (1, 39)=5.428 *p<0.0.5, Time F (3.691, 144.0)=4.539 p=0.0023, Interaction F (5, 195)=0.6146 9=0.6888) (F) Extinction Day 5 lever presses per 10 min time bins (Group F (1, 39)=5.232 *p<0.05, Time F F (3.524, 137.4)=3.115 p=0.0218, Interaction F (5, 195)=2.802 p=0.0181).
Figure 6—figure supplement 1—source data 1. Behavioral data for Figure 6—figure supplement 1.