Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | WT C57BL/6J Mice | The Jackson Laboratory | N/A | |
| Antibody | Anti-Lucifer yellow (rabbit polyclonal) | Invitrogen | Cat #A5750; RRID:AB_1501344 | IF (1:500) |
| Antibody | Anti-gephyrin (mouse monoclonal) | Synaptic System | Cat# 147111; RRID:AB_887719 | IF (1:500) |
| Antibody | PSD-95 MAGUK scaffolding protein (mouse monoclonal) | Neuromab | Cat# 75-028; RRID:AB_2292909 | IF (1:500) |
| Antibody | Anti-choline acetyltransferase (goat polyclonal) | Millipore | Cat# AB144P; RRID:AB_2079751 | IF (1:500) |
| Antibody | Anti-mouse-Dylight 405 (donkey polyclonal) | Jackson ImmunoResearch | Cat# 715-475-150; RRID:AB_2340839 | IF (1:1000) |
| Antibody | Anti-mouse-Alexa 647 (donkey polyclonal) | Jackson ImmunoResearch | Cat# 715-605-151; RRID:AB_2340863 | IF (1:1000) |
| Antibody | Anti-mouse IgG, Fc_Subclass 1 Specific-Dylight 405 (goat polyclonal) | Jackson ImmunoResearch | Cat# 115-475-205; RRID:AB_2338799 | IF (1:1000) |
| Antibody | Anti-mouse moncolonal IgG, Fc_Subclass 2a Specific-Alexa 647 (goat polycolonal) | Jackson ImmunoResearch | Cat# 115-605-206; RRID:AB_2338917 | IF (1:1000) |
| Antibody | Anti-rabbit-Alexa 488 (donkey polyclonal) | Jackson ImmunoResearch | Cat# 711-545-152; RRID:AB_2313584 | IF (1:1000) |
| Antibody | Anti-goat IgG (H+L)-Alexa 647 (donkey polyclonal) | Jackson ImmunoResearch | Cat# 705-605-147; RRID:AB_2340437 | IF (1:1000) |
| Antibody | Streptavidin 488 conjugate antibody | Molecular Probes | Cat# S32354; RRID:AB_2315383 | IF (1:400) |
| Chemical compound, drug | Ames’ Medium | United States Biological | Cat# A1372-25 | |
| Chemical compound, drug | Vectashield | Vector Laboratories | Cat# H-1000; RRID:AB_2336789 | |
| Chemical compound, drug | Red Retrobeads | Lumafluor | https://lumafluor.com/information | |
| Chemical compound, drug | Lucifer yellow CH dilithium salt | Sigma | Cat# L0259 | |
| Chemical compound, drug | Biocytin | Invitrogen | Cat# B1592 | |
| Software, algorithm | Amira | Thermo Fisher Scientific | https://www.fei.com/software/amira-avizo/; RRID:SCR_014305 | |
| Software, algorithm | Bassoon | Harris, 2022 | https://doi.org/10.5281/zenodo.6757605; RRID:SCR_023333 | |
| Software, algorithm | Igor Pro | Igor Pro | RRID:SCR_000325 | |
| Software, algorithm | ImageJ | NIH |
https://imagej.nih.gov/ij/; RRID::SCR_003070 |
|
| Software, algorithm | Imaris | Bitplane |
http://www.bitplane.com/; RRID:SCR_007370 |
|
| Software, algorithm | MATLAB | MathWorks |
https://www.mathworks.com/products/matlab.html; RRID:SCR_001622 |
|
| Software, algorithm | Meshmapper | Paul Bourke | http://paulbourke.net/dome/meshmapper/ | |
| Software, algorithm | ObjectFinder | Della Santina et al., 2013 | https://github.com/lucadellasantina/ObjectFinder; https://zenodo.org/record/4767847; RRID:SCR_023319 | |
| Software, algorithm | Psychopy | Open Science Tools Ltd. Peirce, 2007 | https://psychopy.org/about/index.html | |
| Software, algorithm | ScanImage | MBF Bioscience | https://www.mbfbioscience.com/products/scanimage | |
| Software, algorithm | StreamPix | NorPix | https://www.norpix.com/products/streampix/streampix.php | |
| Software, algorithm | Symphony and Stage | Cafaro, 2019 | https://github.com/Symphony-DAS/symphony-matlab; https://github.com/Stage-VSS/stage-v1 | |
| Software, algorithm | VolumeCut | Della Santina et al., 2021; Della Santina, 2021 | https://github.com/lucadellasantina/VolumeCut; https://doi.org/10.5281/zenodo.5048331 |