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. 2023 Mar 17;12:e80768. doi: 10.7554/eLife.80768

Figure 4. Hair follicles (HFs) dystrophy, melanin clumping, and apoptosis in normal human scalp HF ex vivo co-cultured with ILC1lc and CD8+/NKG2D+ cells.

Figure 4.

(A–C) H&E staining revealed undifferentiated and prominent matrix cells, condensed dermal papilla, and the appearance of apoptotic cells, N=15–19 HFs/group from three independent donors. (D–G) Masson-Fontana histochemistry revealed melanin clumping and ectopic location of melanin granules only in HFs co-cultured with CD8+/NKG2D+and ILC1lc, but not in HFs cultured with PBMCs/PHA. N=7–11, HFs/group from three independent donors. Following Shapiro-Wilk test,Student’s t-test: *p<0.05, **p<0.01, ***p<0.001. (H–K) HFs co-cultured with ILC1lc or CD8+/NKG2D+ cells showed a significantly decreased proliferation (pink, arrowhead) and increased apoptosis (green, wide arrows). N=6 HFs/group from two independent donors, three areas were evaluated per section. Following Shapiro-Wilk test, Student’s t-test: *p<0.05, **p<0.01, ***p<0.001 in the anagen hair bulb compared to HFs cultured with PBMCs/PHA. Scale bars, 50 µm. DP - dermal papilla, HM - hair matrix.

Figure 4—source data 1. Quantitative data for HFs dystrophy, melanin clumping and apoptosis in normal human scalp HF ex vivo co-cultured with ILC1lc and CD8+/NKG2D+ cells.