Skip to main content
. 2023 Mar 17;12:e80768. doi: 10.7554/eLife.80768

Figure 6. Transition of anagen to catagen hair follicles (HFs) following culture with ILC1lc or CD8+/NKG2D+ cells in human scalp HF ex vivo.

Figure 6.

(A) These immune cells significantly accelerated the transformation of anagen HFs into catagen HFs ex vivo compared to ILC2, ILC3, PBMCs/PHA, and neutralizing anti- IFN-γ, anti-NKG2D antibodies. N=28–34 HFs/group taken from six independent donors, Student’s t-test: *p<0.05, **p<0.01, ***p<0.001. (B) ELISA analysis revealed increased IFN-γproduction by ILC1lc /HFs compared to production by CD8+/NKG2D+ cells, ILC2s, ILC3s, and PBMCs/PHA. N=6 healthy donors, 6 × 106 cells from each donor. Following Shapiro-Wilk test, Student’s t-test: *p<0.05, **p<0.01, ***p<0.001. (C) FACS analysis revealed a significant increased intracellular IFN-γexpression in ILC1lc co-cultured with HFs compared to the effector CD8+/NKG2D+and to ILC2s and ILC3s, N=6 blood donors, 1.5 × 106 cells/blood donor. Student’s t-test, p<0.05.

Figure 6—source data 1. Quantitative data for transition of anagen to catagen HFs following culture with ILC1lc or CD8+/NKG2D+ cells in human scalp HF ex vivo.