| Antibodies |
| Rabbit polyclonal anti- FOXG1 |
Abcam |
Cat# ab18259; RRID:AB_732415 |
| Mouse polyclonal anti-NKX2.1(TTF-1) |
SCBT |
Cat# sc-13040, RRID:AB_793532 |
| Rabbit polyclonal anti-Sox6 |
Millipore |
Cat# AB5805, RRID:AB_2302618 |
| Rabbit polyclonal anti-Pan-DLX |
A kind gift from Dr. Morozov at Yale University |
|
| Mouse monoclonal anti-PAX6 |
DSHB |
Cat# pax6, RRID:AB_528427 |
| Mouse monoclonal anti-Nestin |
BD Biosciences |
Cat# 611658, RRID:AB_399176 |
| Mouse monoclonal anti-NeuN |
Millipore |
Cat# MAB377, RRID:AB_2298772 |
| Recombinant Anti-Ki67 antibody |
Abcam |
Cat# ab16667, RRID:AB_302459 |
| Rabbit polyclonal anti-GABA |
Immunostar |
Cat# 20094, RRID:AB_572234 |
| Human-specific NCAM-Alexa594 |
SCBT |
Cat# sc-106, RRID:AB_627128 |
| Human-specific Nucleus |
Millipore |
Cat# MAB1281, RRID: AB_94090 |
| Rabbit polyclonal anti-VGAT |
Synaptic system |
Cat# 131 003, RRID:AB_887869 |
| Mouse monoclonal anti-Gephyrin |
SCBT |
Cat# sc-25311, RRID:AB_627670 |
| Rabbit polyclonal anti-Synaptophysin |
Thermo Fisher Scientific |
Cat# PA1-1043, RRID:AB_2199026 |
| Mouse monoclonal anti-PSD95 |
UC Davis/NIH NeuroMab |
Cat# 75-028, RRID:AB_2292909 |
| Rabbit polyclonal anti- Tubulin β-3 |
Biolegend |
Cat# 802001, RRID:AB_2564645 |
| Rabbit polyclonal anti- Calbindin D |
Swant |
Cat# CB38, RRID:AB_10000340 |
| Goat polyclonal anti- Somatostatin |
SCBT |
Cat# sc-7819, RRID:AB_2302603) |
| Rabbit Polyclonal anti-parvalbumin antibody |
Swant |
Cat# PV 25, RRID:AB_10000344 |
| Rabbit Polyclonal anti-VIP |
Immunostar |
Cat# 20077, RRID:AB_572270 |
| Rabbit anti-LHX6 |
A kind gift from Dr. Pachnis at the Crick Institute |
|
| Mouse monoclonal anti-GFAP |
UC Davis/NIH NeuroMab Facility |
Cat# 73-240, RRID:AB_10672298 |
| Rabbit Polyclonal anti-TH |
Pel-Freez |
Cat# P40101-150, RRID:AB_2617184 |
| Rabbit Polyclonal anti-DARPP32 |
SCBT |
Cat# sc-11365, RRID:AB_639000 |
| Goat Polyclonal anti-CHAT |
Chemicon |
Cat# AB144, RRID:AB_90650 |
| Rabbit monoclonal anti-TBR2 |
Abcam |
Cat# ab31940, RRID:AB_2200219 |
| Rabbit Polyclonal anti-5HT |
Immunostar |
Cat# 20080, RRID:AB_572263 |
| Rabbit Polyclonal anti-ZNT3 |
Alomone Lab |
Cat# AZT-013, RRID:AB_2756813 |
| Rabbit Polyclonal anti-IBA1 |
FUJIFILM Wako |
Cat# 019-19741, RRID:AB_839504 |
| Rabbit Monoclonal anti-cleaved-caspase |
Cell Signaling |
Cat# 9661, RRID:AB_2341188 |
| Mouse Monoclonal anti-CAMKII |
Millipore |
Cat# 05-532, RRID:AB_309787 |
| Rabbit Polyclonal anti- OCT4 |
Cell Signaling |
Cat# 2750, RRID:AB_823583 |
| Rabbit Polyclonal anti-KCC2 |
Abcam |
Cat# ab97502 RRID: AB_10866258
|
| Bacterial and virus strains |
| EnvA G-Deleted Rabies-mCherry |
Salk Institute for Biological Studies |
Addgene 32636 |
| Biological samples |
| Chemicals, peptides, and recombinant proteins |
| Geltrex |
Thermo Fischer Scientific |
Cat#A1413201 |
| Y27632 |
ApexBio |
Cat# A3008 |
| β-Mercaptoethanol |
Thermo Fischer Scientific |
Cat# 21985023 |
| LDN-193189 |
Selleck Chem |
Cat# S2618 |
| SB431542 |
Tocris Bioscience |
Cat# 1614 |
| SAG |
Selleck Chem |
Cat# S7779 |
| IWP-2 |
Selleck Chem |
Cat# S7085 |
| N-2 Supplement |
Life Technologies |
Cat#17502048 |
| L-Ascorbic acid |
Sigma-Aldrich |
Cat# A8960-5G |
| FGF8 |
Prospecbio |
Cat# cyt-839 |
| GDNF |
Prospecbio |
Cat# cyt-305 |
| BDNF |
Prospecbio |
Cssat# cyt-207 |
| B27 Supplement |
Thermo Fischer Scientific |
Cat# 17504001 |
| CultureOne |
Thermo Fischer Scientific |
Cat# A3320201 |
| DAPT |
Sigma-Aldrich |
Cat# D5942 |
| PD033299 |
Sigma-Aldrich |
Cat# PZ0199 |
| Poly-L-Ornithine |
Sigma-Aldrich |
Cat# P4957 |
| Fibronectin |
Thermo Fischer Scientific |
Cat# J66903 |
| Paraformaldehyde |
Electron Microscopy science |
Cat# NC0179595 |
| DAPI |
Invitrogen |
Cat# 10236276001 |
| Fluoromount-G |
SouthernBiotech |
Cat# 0100-01 |
| Pilocarpine hydrochloride |
Sigma-Aldrich |
Cat# P6503 |
| Diazepam |
Henry Schein |
Cat# 1278188 |
| Kainic acid |
Hello Bio |
Cat# HB0355 |
| Sucrose |
Boston Bioproduct |
Cat# P-908 |
| Trehalose |
Sigma-Aldrich |
Cat# A9165 |
| Boc-Asp (OMe)-fluoromethyl ketone |
Sigma-Aldrich |
Cat# B2682 |
| Critical commercial assays |
| Deposited data |
| Experimental models: Cell lines |
| human iPSC 317 |
Shao et al.,2019
|
|
| Human H9 ESCs |
Wicell |
Wae009-A |
| Experimental models: Organisms/strains |
| NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl>/SzJ |
The Jackson Laboratory |
Strain #:005557 RRID:IMSR_JAX:005557 |
| Oligonucleotides |
| Recombinant DNA |
| pBOB-synP-HTB |
Addgene |
Cat# 30195 |
| pLenti-hSyn-eNpHR3.0-EYFP |
Addgene |
Cat# 26775 |
| pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE |
Addgene |
Cat# 20945 |
| Software and algorithms |
| AcqKnowledge 4.0 EEG Acquisition and Reader Software |
BIOPAC Systems Inc |
Cat# UPGACQ |
| Anymaze video-tracking system |
Stoelting, Wood Dale, IL |
Version 5 |
| Sirenia® Software |
Pinnacle technology |
Sirenia version 2.2.5. |
| Ethovision software |
Noldus Technology |
EthoVision XT 15.0.1418 |
| Image J |
NIH |
ImageJ version 1.53 |
| IMARIS software |
Bitplane |
Cat# BPI-IM90-SF-N |
| GraphPad Prism |
Graph Pad |
GraphPad Prism version 8.0.0 |
| Clampex software |
Molecular Devices |
Version 10 and 11 |
| Clampfit software |
Molecular Devices |
Version 10 |
| StereoInvestigator |
MBF Bioscience |
SS-15 |
| PASS software |
NCSS, LLC |
Version 12.0.5 |
| Other |
