Figure 1.

Macromer synthesis and Nor-Tet hydrogel polymerization. (a) HA macromers modified with Nor (HANor) or (b) Tet (HATet) (c) spontaneously from stable crosslinked hydrogels when combined. (d) Representative time sweep rheology plot of 2% w/v Nor-Tet hydrogels polymerizing at 37 °C for 60 minutes and 180 minutes. (e) HANor and HATet macromers can be dissolved rapidly at room temperature and loaded into syringes for mixing through a Luer-Lock coupler. Mixed macromers remain in solution long enough for extrusion into (f) molds or (g) cavities, resulting in self-forming hydrogels that conform to the shape of space they occupy.