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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Brain Behav Immun. 2023 Jan 19;109:204–218. doi: 10.1016/j.bbi.2023.01.009

Figure 3. Treadmill exercise reduces the burden of Aβ in the hippocampal region of Tg-mice.

Figure 3.

Six-month-old Tg-mice (n=6/group) were allowed to gently run on the treadmill. Following the treadmill exercise, Aβ levels were examined in hippocampal homogenates of different groups of mice by Western Blot using the 82E1 monoclonal antibody (A). Actin was used as the loading control. All the protein bands were scanned and densitometric analysis representing mean ± SD for Aβ levels relative to Non-Tg controls. (B) Quantification of Aβ level in protein bands indicates - ***p<0.001 (=0.0005) vs Non-Tg mice and ***p<0.001(=0.0008) vs Tg-mice with treadmill exercise. (C) Diaminobenzidine staining of hippocampal sections were performed using the monoclonal 82E1 antibody for demonstrating the Aβ pathology in cortex and hippocampus region of Tg-mice with and without treadmill exercise. The Aβ plaque pathology was characterized for number of plaques (D), average size of plaques (E) and density of plaques (F). Results are mean ± SD of six per group. All the quantification of Aβ plaques was performed using the Image J. Statistical analysis were conducted by using One-way ANOVA followed by Tukey’s multiple comparison tests. The number of plaques in Cortex - ***p<0.001 (=2.8943×10−5) vs non-Tg mice; ***p<0.001(=0.0005) vs Tg-mice with exercise and in hippocampus - ***p<0.001(=6.2981×10−5) vs non-Tg mice; ***p<0.001(=0.0008) vs Tg-mice with exercise. The size of plaques - ***p<0.001 (=6.1500×10−9) vs non-Tg mice; ***p<0.001 (=2.44×10−13) vs Tg-mice with exercise and density of plaques - ***p<0.001(=2.6173×10−49) vs non-Tg mice; ***p<0.001(=9.1780×10−19) vs Tg-mice with exercise. ns – Non-significant.