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. Author manuscript; available in PMC: 2024 Mar 13.
Published in final edited form as: Curr Biol. 2023 Jan 23;33(5):791–806.e7. doi: 10.1016/j.cub.2022.12.059

Figure 3: The centrosome is encased by the centriculum.

Figure 3:

(A-E) The spatial relationship between the centriculum (detected using mCherry::SP12 or SP12::GFP) and the following components of the centrosome in a 1-cell stage embryo at metaphase: GFP::SAS-6 (strain OCF124, n=6 centrosomes), SPD-2::mCherry (OCF127, n=10 centrosomes), GFP::SPD-5 (OCF176, n=6 centrosomes), GFP::AIR-1 (OCF158, n=16 centrosomes) and GFP::TBA-2 (MSN146, n=8 centrosome). Representative images are shown. Scale bar = 5 μm. Graphs show the fluorescence intensities along lines (Figure S5A) that traverse the centrosomal protein (blue) or the centriculum (orange). Error (in grey) around the mean represents 95% confidence interval. A.U. = arbitrary units. For related data see Figure S5.