Figure 8: Ir93a promotes oviposition site attraction in Ae. aegypti and is conserved among arthropods.

(A) Oviposition site selection assay. (B) Potential oviposition site containers at assay end, with mosquitoes and eggs present only in wild type, wet container. (C) Upper panel, egg deposition in wet containers by assay end. Lower panel, photograph of portion of oviposition substrate from wet container. (D) Quantitation of egg deposition in seeking assay. 70 gravid females/assay. wild type (LVP), n=8 assays. AaIr93a^RFP/AaIr93a^RFP, n=7. AaIr93a^EYFP/AaIr93a^EYFP, n=8. Letters denote distinct groups, comparisons among wet containers. Steel-Dwass p≤0.01. (E) Mature oocytes present in dissected ovaries of gravid females. Letters denote distinct groups, Steel-Dwass p≤0.02. (F) Egg-laying ability assay. (G) Eggs laid by single gravid females placed in a water-containing oviposition chamber. n=20 individuals/genotype. Kruskal-Wallis, p =0.18. (H) Ionotropic Receptor distribution. Cladogram, land colonization and blood-feeding emergence times based on ^{33,59,67–70}. Cam, Cambrian. Ord, Ordovician. Si, Silurian. Dev, Devonian. Car, Carboniferous. Per, Permian. Tri, Triassic. Jur, Jurassic. Cre, Cretaceous. Cen, Cenozoic (era). Brackets note a disease transmitted by each vector. See also Figure S6.